Mouse IL-17E/IL-25 ELISA Kit Affinity PurificationSpecifications Name Mouse IL 17E IL 25 ELISA Kit Hgnc ID 13765 Specie mouse Assay Type sandwich Assay Time 3. 5 h Sample Type Serum, plasma, cell culture supernatant, and other biological samples Sample Amount 10 L Sensitivity 1. 22 pg mL Range Standard 62. 5 pg mL 4000 pg mL Recovery 73% 99% Average Recovery 83% Intra Precision 3. 2% 3. 6% Inter Precision 3. 8% 4. 5% Plate Type Detachable 96 well plate Platform ELISA Components 96 well polystyrene
17400131 17400132 17400133 17400134 17400135 17400037 17400021 17400073 17400553 17400696 17100767 Accessories GL32
The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase
check sample recovery (if ERC included)
Process immediately or freeze: To prevent nicking by residual DNase
Feature LB Lennox LB Miller NaCl content 5 g/L 10 g/L Osmolarity Lower Higher Use Case Salt-sensitive applications Standard E
TMB substrate incubation: 15 minutes at room temperature
where NAT sensitivity targets include 10 CFU/mL to replace culture methods and 100 CFU/mL to replace indicator cell culture methods
48 cm Buffer System Tris-Glycine buffer system Maximum Sample Loading Volume 50 μL Recommended Sample Loading Volume 25 μL Storage
The kit is intended for testing cell culture samples (≤10^7 cells)
938 - 700 pg/mL Recovery 81% - 119% Average Recovery 101% Intra Precision 3
Whether used for routine microbial culture
It is a basic protein containing a nuclear localization signal
